【文獻(xiàn)標(biāo)題】Proline with or without Hydroxyproline Influences Collagen Concentration and Regulates Prolyl 4-Hydroxylase α (I) Gene Expression in Juvenile Turbot (Scophthalmus maximus L.)
【作者】ZHANG Kaikai，MAI Kangsen，XU Wei，et.al
【作者單位】中國海洋大學(xué)（Ocean University of China）
【文獻(xiàn)中引用產(chǎn)品】
試劑L-脯氨酸
試劑L-羥脯氨酸
【關(guān)鍵詞】proline; hydroxyproline; juvenile turbot; high plant protein; collagen; prolyl 4-hydroxylase α(I)
【DOI】DOI 10.1007/s11802-015-2436-0
【影響因子(IF)】1.30
【出版期刊】《Journal of Ocean University of China》
【產(chǎn)品原文引用】
Experimental Diets
L-Pro (content>99%) andL-Hyp (content>99%) were obtained from Hengyuan Biotech. Co., Ltd (Shanghai,China). White fish meal (WFM), soybean meal (SBM), and wheat gluten meal (WGM) were used as the major protein sources, fish oil as the major lipid source and wheat flour as the carbohydrate source. Lysine-H2SO4, DL-Methionine, L-Threonine, L-Arginine, L-Isoleucine, L-Leucine, and L-Valine (crystalline amino acids) were supplemented to meet the essential amino acids requirements of juvenile turbot based on the whole body amino acid profile (Kaushik, 1998). The basal diet with 50% crude protein and 12% crude lipid was the same as that we formulated in our previous study (Zhang et al., 2013). The other two protein and lipid contents identical experimental diets were formulated by supplementing the basal diet with either 0.75% Pro or 0.75% Pro and 0.75% Hyp, which were named as Pro-0.75 and Pro+Hyp, respectively. The content of L-Alanine was adjusted in order to maintain an equal nitrogen concentration in all diets (Table 1). The final content of dietary Pro was 3.22%, 3.88% and 3.82%, respectively, while the final content of Hyp was 0.12%, 0.22%, and 0.67%, respectively. The amino acid composition of the experiment diets was shown in Table 2.
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