人胎球蛋白A（FETU-A）

試劑名稱：人胎球蛋白A（FETU-A）

英文名稱：Human Fetuin A,FETU-A ELISA KIT

檢測范圍：詳見說明書

產(chǎn)品規(guī)格：96人份/48人份

檢測目標(biāo)：人、大小鼠、豬、豚鼠、雞、羊、兔...

保存條件： 2-8℃

應(yīng)用范圍：科研用檢測試劑

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Assay procedure（操作步驟）
1.Dilute and add sample:Dilute Original density Standard as follow table:
8 pg/ml 5 Standard 150μl Original density Standard+150μl Standard diluent
4pg/ml 4 Standard 150μl 5 Standard+150μl Standard diluent
2 pg/ml 3 Standard 150μl 4 Standard+150μl Standard diluent
1 pg/ml 2 Standard 150μl 3 Standard +150μl Standard diluent
0.5 pg/ml 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample：Set blank wells separay （blank comparison wells don’t add sample and HRP-
Conjugate reagent, other each step operation is same）. testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl （sample final
dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as
possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold （or 20-fold） with distilled
water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction（the blue color
change to yellow color）.
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution
and within 15min. 

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